WNT2 anticorps (Internal Region)

N° du produit ABIN1049474

Détail du produit anti-WNT2 anticorps

Antigène: WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))

Épitope: Internal Region

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WNT2 est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human WNT2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%) Marmoset, Mouse, Hamster, Guinea pig, Platypus (87%) Galago, Rat, Ferret, Elephant, Panda, Dog, Cat, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Armadillo (80%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 15 amino acid peptide from internal region of human WNT2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey (100%), Marmoset, Mouse, Hamster, Guinea pig, Platypus (87%), Galago, Rat, Ferret, Elephant, Panda, Dog, Cat, Bat, Horse, Rabbit, Pig, Turkey, Chicken, Armadillo (80%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur WNT2

Antigène: WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))

Autre désignation: WNT2 / IRP (WNT2 Produits)

Synonymes: anticorps CG1916, anticorps D-wnt-2, anticorps DWnt-2, anticorps DWnt2, anticorps Dm DWnt2, anticorps Dm-2, anticorps Dmel\\CG1916, anticorps Dwnt-2, anticorps Wnt, anticorps Wnt-2, anticorps dWnt2, anticorps wnt2, anticorps WNT2, anticorps irp, anticorps Xwnt2, anticorps wnt-2, anticorps Xwnt-2, anticorps int1l1, anticorps 2610510E18Rik, anticorps Int1l1, anticorps Irp, anticorps Mirp, anticorps Wnt2a, anticorps INT1L1, anticorps IRP, anticorps ZfWnt2, anticorps Wnt oncogene analog 2, anticorps Wnt family member 2, anticorps wingless-type MMTV integration site family member 2, anticorps wingless-type MMTV integration site family, member 2, anticorps Wnt2, anticorps wnt2, anticorps WNT2

Sujet: Name/Gene ID: WNT2
Family: WNT

Synonyms: WNT2, IRP, Int-1-like protein 1, Int-1-related protein, Protein Wnt-2, Secreted growth factor, INT1L1

ID gène: 7472

Pathways: Signalisation WNT