p21 anticorps

N° du produit ABIN105216

N° du produit (Fournisseur): 200-301-171

Cet anticorps anti-p21 est un anticorps Souris Monoclonal détectant p21 dans WB, IHC, ELISA, IF, IP, MA et FM. Adapté pour Humain. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour p21 anticorps (ABIN105216)

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp p21 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Clone: WA-1

Détail du produit anti-p21 anticorps

N° du produit (Fournisseur): 200-301-171

Fournisseur: Rockland

Fonction: p21 WAF1 Antibody

Réactivité croisée (Details): This protein A purified mouse monoclonal antibody reacts with human and mammalian p21. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1).

Attributs du produit: Synonyms: mouse anti-p21/WAF1 antibody, Cyclin-dependent kinase inhibitor 1A (p21, CIP1) antibody, DNA Synthesis Inhibitor antibody, MDA 6 antibody, MDA6 antibody, Melanoma Differentiation Associated Protein 6 antibody

Purification: Protein A purified

Stérilité: Sterile filtered

Immunogène:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-p21/WAF1 has been tested in ELISA, western blot, and immunofluorescence. This product is also suitable for immunohistochemistry. The antibody detects a 21 kDa band corresponding to the p21 protein.  MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry.   Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining).  In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein.  This antibody immunoprecipitates monomeric p21WAF1 and not p21 complexes with other proteins. High level expression of p21WAF1 requires induction of wild-type p53 expression (e.g. via exposure to DNA damaging agents).

Western Blot Dilution: 1:2,000 - 1:10,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:250

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-p21 (ABIN105216)

Supiot, Hill, Bristow: "Nutlin-3 radiosensitizes hypoxic prostate cancer cells independent of p53." dans: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 993-9, (2008) (PubMed).

Joshi, Chen, Kalemkerian, Adil, Kraut, Sarkar: "Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer." dans: Cancer gene therapy, Vol. 5, Issue 3, pp. 183-91, (1998) (PubMed).

Kagawa, Fujiwara, Hizuta, Yasuda, Zhang, Roth, Tanaka: "p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells." dans: Oncogene, Vol. 15, Issue 16, pp. 1903-9, (1997) (PubMed).

Yang, Perkins, Ohno, Nabel, Nabel: "The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo." dans: Nature medicine, Vol. 1, Issue 10, pp. 1052-6, (1996) (PubMed).

Waldman, Kinzler, Vogelstein: "p21 is necessary for the p53-mediated G1 arrest in human cancer cells." dans: Cancer research, Vol. 55, Issue 22, pp. 5187-90, (1995) (PubMed).

Yeh, Shatkin: "A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21." dans: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15815-20, (1995) (PubMed).

Détails sur p21

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: CDKN1A

Sujet: Background: p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA. Anti-p21WAF1 antibody is useful for researches interested in studying tongue and gastrointestinal cancers, as well as, ubiquitin and cyclin binding proteins.

ID gène: 1026

NCBI Accession: NP_510867

UniProt: P38936

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy