RAD9A anticorps (pSer1129)

N° du produit ABIN106404

N° du produit (Fournisseur): 600-401-345

Cet anticorps anti-RAD9A est un anticorps Lapin Polyclonal détectant RAD9A dans WB et ELISA. Adapté pour Saccharomyces cerevisiae.

Aperçu rapide pour RAD9A anticorps (pSer1129) (ABIN106404)

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD9A est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-RAD9A anticorps

Épitope: pSer1129

N° du produit (Fournisseur): 600-401-345

Fournisseur: Rockland

Fonction: Rad9 phospho S1129 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against the phosphorylated form of yeast Rad9 at the pS1129 residue.

Attributs du produit: Synonyms: Rabbit anti-Rad9 phospho antibody, Rabbit anti-Rad9 pS1129 antibody, Rad 9, Rad-9, Cell cycle checkpoint control protein antibody, Cell cycle checkpoint control protein RAD9A antibody, DNA repair exonuclease rad9 homolog A antibody, DNA repair protein RAD9, YDR217C, YD9934.02C

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 1120-1145 from the aa1309 yeast Rad9 protein conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This phospho specific polyclonal antibody was tested by ELISA.  Data from ELISA indicates the antibody is reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. No reactivity is expected against the human or mouse analogs of RAD9.  Reactivity against RAD9 from other sources is unknown.   Although not tested, this antibody is likely functional by WB, IHC and IP. This product has been assayed against 0.1 μg of phosphorylated peptide (pS1129) in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) code # TMBE-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 is suggested for this product.  Minimal reactivity was detected against the non-phosphorylated form (S1129) of the immunizing peptide. This antibody appears to be specific for the active form (phosphorylated) of the protein.  Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.43 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur RAD9A

Antigène: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Autre désignation: RAD9

Sujet: Background: Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9.  Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions.  Another cellular strategy is to delay cell-cycle transitions at multiple points.  The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G₂/M arrest after irradiation with X-rays.  Subsequently, it has become clear that Rad9 also functions at the G₁/S, intra-S and mid-anaphase checkpoints.  Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR).  Rad9 may also have a pro-apoptotic function.  This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells.  Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p, is hyperphosphorylated by Mec1p and Tel1p, and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148 kDa.  The human homolog is reported at 48.5 kDa.

ID gène: 851803

NCBI Accession: NP_010503

UniProt: P14737

Pathways: Positive Regulation of Response to DNA Damage Stimulus