Superoxide Dismutase anticorps

N° du produit ABIN107158

Détail du produit

Antigène: Superoxide Dismutase

Reactivité: Boeuf (Vache), Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.

Pureté: Anti-SUPEROXIDE DISMUTASE (SOD) is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Superoxide Dismutase [Bovine erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: SUPEROXIDE DISMUTASE (SOD) Antibody has been assayed against 1.0 µg of Superoxide Dismutase [Bovine erythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10.000 to 1:50.000 is suggested for this product.
ELISA Dilution: 1:10.000 - 1:50.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détail du antigène

Antigène: Superoxide Dismutase

Sujet: Synonyms: ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody, SOD

ID gène: 281495

UniProt: P00442