RIF1 anticorps (AA 2432-2444)

N° du produit ABIN1103996

Détail du produit anti-RIF1 anticorps

Antigène: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Épitope: AA 2432-2444

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RIF1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Human RIF1. This antibody is expected to recognize both reported isoforms (NP_060621.3, NP_001171134.1). The immunizing peptide sequence has been optimised for cross-reactiviy in mouse. Reported variants represent identical protein: NP_001171135.1, NP_001171136.1, NP_001171134.1

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%) Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-RNLQSRWRSPSHEN from the C-terminus of human RIF1 (NP_060621.3, NP_001171134.1). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Xenopus (93%), Galago, Rat, Panda, Bovine, Dog, Porcine, Guinea pig (86%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: Peptide-ELISA (1:8000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 270kD band observed in lysates of cell line HeLa and of Mouse Embryonic Stem cells (calculated MW of 274kD according to NP_060621.3). The observed band is suppressed by introduction of Rif1 siRNA to HeLa. Recommended concentration: 1-3 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur RIF1

Antigène: RIF1 (RAP1 Interacting Factor Homolog (RIF1))

Autre désignation: RIF1 (RIF1 Produits)

Synonymes: anticorps 5730435J01Rik, anticorps 6530403D07Rik, anticorps AU016181, anticorps AW549474, anticorps D2Ertd145e, anticorps RGD1562474, anticorps fc38c03, anticorps wu:fc38c03, anticorps si:dkey-89p3.2, anticorps replication timing regulatory factor 1, anticorps RAP1 interacting factor homolog (yeast), anticorps RIF1, anticorps Rif1, anticorps rif1

Sujet: Name/Gene ID: RIF1

Synonyms: RIF1

ID gène: 55183

NCBI Accession: NP_060621, NP_001171134

UniProt: Q5UIP0

Pathways: Stem Cell Maintenance