CD5 anticorps (PerCP)

N° du produit ABIN1112198

Détail du produit anti-CD5 anticorps (PerCP)

Antigène: CD5

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD5 est conjugé à/à la PerCP

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Monoclonal Mouse Anti-Human CD5 PerCP, is recommended for use in flow cytometry for identification of T lymphocytes CD5+.

Clone: 33-1C5

Isotype: IgG2a

Information d'application

Indications d'application: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Commentaires:

Peridin-cholophyll-protein complex (Prozyme).

Prélèvement de l'échantillon: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD5 PerCP and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PerCP-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Stock: 4 °C

Détails sur CD5

Antigène: CD5

Autre désignation: CD5 (CD5 Produits)

Synonymes: anticorps CD5, anticorps Ly-1, anticorps Ly-12, anticorps Ly-A, anticorps Lyt-1, anticorps LEU1, anticorps T1, anticorps CD5 molecule, anticorps CD5 antigen, anticorps CD5 molecule like, anticorps Cd5 molecule, anticorps CD5, anticorps Cd5, anticorps CD5L

Sujet: This antibody identifies a 67 kDa lymphocyte surface antigen belonging to the CD5 cluster on peripheral blood T lymphocytes, 70% of thymocytes and a limited number of malignancies of T cell origin. The CD5 antigen is expressed on the majority of malignant B CLL, T CLL and T ALL cells and a proportion of B lymphoma cells.