MBP Tag anticorps

N° du produit ABIN1112992

Détail du produit

Antigène: MBP Tag

Hôte: Souris

Conjugué: Inconjugué

Application: Western Blotting (WB)

Immunogène: Recombinant MBP protein with full length.

Isotype: IgG1

Information d'application

Indications d'application: WB (1:1000-10,000). Other applications have not been tested. The optimal dilutions should be determined by end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.

Détail du antigène

Antigène: MBP Tag

Classe de substances: Tag

Sujet: Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.