ITGA5 anticorps

N° du produit ABIN111905

Détail du produit anti-ITGA5 anticorps

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGA5 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Detects human granulocytes, monocytes and platelets. X-6 detects an antigen of 154-180 kDa by immunoprecipitation. Antigen distribution on Isolated cells and Tissue sections: Granulocytes, monocytes. Positive on blood monocyte-derived macrophages days 0-28. Dull staining with 95 % cord blood CD34 cells. Weak reaction on resting neutrophils. Staining increase with maturation of granulocytes. Treatment with fMLP, TNF, GM-CSF and temperature causes postive shift with granulocytes and monocytes. PMA treatment of granulocytes leads to decrease in no. of positive cells. X-6 is positive on lining cells in different infiltrations and endothelial cells in the synovial membrane of RA patients. The staining pattern is similar to CD34 in these patients.

Purification: Affinity chromatography.

Immunogène: Human monocytes.

Clone: X-6

Isotype: IgG1

Information d'application

Indications d'application: Suitable for Immunohistochemistry on frozen sections (2 μg/mL (1: 100) and FACS (use 10 μLstock solution to label 10^6 cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 10 mg/mL bovine serum albumin (BSA) as a stabilizer and 0.01 % Thimerosal as a preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur ITGA5

Antigène: ITGA5 (Integrin, alpha 5 (ITGA5))

Autre désignation: CD49e / ITGA5 (ITGA5 Produits)

Synonymes: anticorps ITGA5, anticorps Cd49e, anticorps Fnra, anticorps VLA5, anticorps CD49e, anticorps FNRA, anticorps VLA5A, anticorps Itga5_retired, anticorps integrin subunit alpha 5, anticorps integrin alpha 5 (fibronectin receptor alpha), anticorps ITGA5, anticorps Itga5

Sujet: The X-6 antibody is useful for the detection of the fibronectin receptor, or CD49e. CD49e is a component of an adhesion receptor heterodimer (α5β1) to fibronectin and invasin. α5β1 recognizes an Arg-Gly-Asp sequence in fibronectin. Binding of fibronectin through α5β1 is important for cell survival and induces apoptosis, particularly in hemopoietic cells.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5

ID gène: 3678

NCBI Accession: NP_002196

UniProt: P08648

Pathways: Integrin Complex