CD44 anticorps

N° du produit ABIN114215

Détail du produit anti-CD44 anticorps

Antigène: CD44

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD44 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts with all isoforms of CD44 (also known as Pgp, Ly-24) as well as both Ly-24.1 and Ly-24.2 (1,4). By Flow Cytometry, the main cellular reactivities are B cells, monocytes, macrophages and variable subsets of thymocytes and peripheral T cells.

Purification: Purified

Clone: IM7-8-1

Isotype: IgG2b

Information d'application

Indications d'application: Flow Cytometry. Immunohistochemistry {both Paraffin (2.5-10 μg/mL) and Frozen sections}. This clone is also reported to work in Immunoprecipitation, ELISA, and complementdepletion (1,2,3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with a cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of ABIN114215. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of a secondary antibody (f. e. FITC Goat anti-rat IgG (H+L) at 1: 500 dilution). 9. Incubate the tubes at 4°C for 30-60 minutes. (protect from light since mostfluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD44

Antigène: CD44

Autre désignation: CD44 (CD44 Produits)

Synonymes: anticorps CDW44, anticorps CSPG8, anticorps ECMR-III, anticorps HCELL, anticorps HUTCH-I, anticorps IN, anticorps LHR, anticorps MC56, anticorps MDU2, anticorps MDU3, anticorps MIC4, anticorps Pgp1, anticorps AU023126, anticorps AW121933, anticorps AW146109, anticorps HERMES, anticorps Ly-24, anticorps Pgp-1, anticorps CD44A, anticorps METAA, anticorps RHAMM, anticorps XCD44, anticorps cd44, anticorps cdw44, anticorps cspg8, anticorps ecmr-iii, anticorps hcell, anticorps lhr, anticorps mc56, anticorps mdu2, anticorps mdu3, anticorps mic4, anticorps mutch-i, anticorps pgp1, anticorps CD44 molecule (Indian blood group), anticorps syndecan 4, anticorps CD44 antigen, anticorps CD44 molecule (Indian blood group) S homeolog, anticorps CD44, anticorps SDC4, anticorps Cd44, anticorps cd44.S

Sujet: CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

ID gène: 12505

NCBI Accession: NP_001034240

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling