EGF anticorps
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- Antigène Voir toutes EGF Anticorps
- EGF (Epidermal Growth Factor (EGF))
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp EGF est non-conjugé
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Application
- ELISA (Capture)
- Specificité
- This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
- Réactivité croisée (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity Chromatograpghy on Protein G.
- Immunogène
- Purified recombinant Human EGF, 6kDa, 53 amino acid residues
- Clone
- S-134
- Isotype
- IgG1
- Top Product
- Discover our top product EGF Anticorps primaire
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- Indications d'application
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ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
- Concentration
- 1.00 mg/mL
- Buffer
- 0.01 M PBS, pH 7.2 without preservatives
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
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- Antigène
- EGF (Epidermal Growth Factor (EGF))
- Autre désignation
- EGF (EGF Produits)
- Synonymes
- anticorps HOMG4, anticorps URG, anticorps AI790464, anticorps CEGF, anticorps epidermal growth factor, anticorps pro-epidermal growth factor, anticorps EGF, anticorps egf, anticorps CpipJ_CPIJ020278, anticorps Egf
- Sujet
- Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
- ID gène
- 1950
- NCBI Accession
- NP_001954
- UniProt
- P01133
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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