IL-8 anticorps
-
- Antigène Voir toutes IL-8 (IL8) Anticorps
- IL-8 (IL8) (Interleukin 8 (IL8))
-
Reactivité
- Humain
-
Hôte
- Souris
-
Clonalité
- Monoclonal
-
Conjugué
- Cet anticorp IL-8 est non-conjugé
-
Application
- Western Blotting (WB), Neutralization (Neut), Enzyme Immunoassay (EIA)
- Purification
- Affinity Chromatography on Protein G.
- Immunogène
- Purified recombinant human IL-8.
- Clone
- I8-60
- Isotype
- IgG1
- Top Product
- Discover our top product IL8 Anticorps primaire
-
-
- Indications d'application
- ELISA: Can be used as a Tracer antibody in Sandwich ELISA for Detection of Human IL-8(matched pair with clone I8-S2, DM1022 as Capture antibody)Suggested Capture Coating Dose: 0.3 μg/well, substrate TMB. Neutralizing: In chemotaxis assay, the antibody inhibited 98.4 % of the chemotacticactivity of IL-8 on RB/293 cell using 5 μg/mL of MAb and 10 ng/mL of Human IL-8 in assay. This MAb inhibited the chemotactic effect of Human IL-8, had no inhibitory effect to MIP1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is specialneutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calciumion changes in human granulocytes using FACS. Western Blot: Concentration of 0.02-0.1 μg/mL MAb will allow visualization of 100 ng/laneof Human IL-8.
- Restrictions
- For Research Use only
-
- Reconstitution
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
- Buffer
- 0.01 M PBS, pH 7.0
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody at 2-8 °C for one month or at -20 °C for longer.
-
- Antigène
- IL-8 (IL8) (Interleukin 8 (IL8))
- Autre désignation
- Interleukin-8 / IL8 (IL8 Produits)
- Synonymes
- anticorps CXCL8, anticorps GCP-1, anticorps GCP1, anticorps LECT, anticorps LUCT, anticorps LYNAP, anticorps MDNCF, anticorps MONAP, anticorps NAF, anticorps NAP-1, anticorps NAP1, anticorps IL-8, anticorps AMCF-I, anticorps il8, anticorps IL8, anticorps si:dkey-151b16.2, anticorps cxcli2, anticorps cxcl8, anticorps gcp-1, anticorps il-8, anticorps lynap, anticorps mdncf, anticorps monap, anticorps nap-1, anticorps C-X-C motif chemokine ligand 8, anticorps interleukin 8, anticorps chemokine (C-X-C motif) ligand 8a, anticorps chemokine (C-X-C motif) ligand 1-like, anticorps chemokine (C-X-C motif) ligand 8 L homeolog, anticorps CXCL8, anticorps il8, anticorps cxcl8a, anticorps IL8, anticorps Cxcl8, anticorps LOC422654, anticorps cxcl8.L
- Sujet
- Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor
- ID gène
- 9606
- UniProt
- P10145
- Pathways
- Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
-