RAF1 anticorps

N° du produit ABIN114553

Cet anticorps Souris Monoclonal détecte spécifiquement RAF1 dans WB et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour RAF1 anticorps (ABIN114553)

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAF1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 410

Détail du produit anti-RAF1 anticorps

Specificité: This antibody reacts to Raf-1 N-terminal (1-240 a.a.)

Purification: Protein G affinity purified

Immunogène: Purified recombinant Raf-1 (mw:75 kDa)

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Western Blot: An antibody concentration of 1 μg/mL will allow visualization of 0.2 g/laneof human Raf-1. A main band (75 kDa) was visible.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/mL

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

Détails sur RAF1

Antigène: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Autre désignation: RAF1

Sujet: The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf

ID gène: 5894

NCBI Accession: NP_002871

UniProt: P04049

Pathways: Signalisation MAPK, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling