IL-1 beta anticorps

N° du produit ABIN115954

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-1 beta dans WB, EIA et Func. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour IL-1 beta anticorps (ABIN115954)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-1 beta est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-IL-1 beta anticorps

Specificité: Reacts with Interleukin-1-beta.

Réactivité croisée (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography using immobilized Rat IL-1 beta matrix.

Immunogène: Highly pure (>98%) recombinant Rat IL-1 beta. Remarks: Molecular Weight: ~150 kDa

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-1 beta (0.20 ng/mL), a concentration of 0.09-0.14 μg/mL of this antibody is required. Sandwich ELISA: To detect Rat IL-1 beta by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with biotinylated Anti-Rat IL-1 beta (PP034B1 or PP034B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant RatIL-1 beta. Western blot: To detect Rat IL-1 beta by Western blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat IL-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at -20 °C for 1 year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 12 months

Détails sur IL-1 beta

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: Interleukin-1 beta / IL-1B

Sujet: IL1-beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1A and IL1B binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1B is a secreted cytokine, IL-1A is predominantly a cell-associated cytokine.Synonyms: Catabolin, IL-1 beta, IL1 beta, IL1B, IL1F2

ID gène: 10116

UniProt: Q63264

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome