TNF alpha anticorps

N° du produit ABIN115973

L’anticorps anti-TNF alpha Polyclonal Lapin est utilisé pour la détection de TNF alpha dans des échantillons de Rat. Il a été validé pour WB, Func et EIA.

Aperçu rapide pour TNF alpha anticorps (ABIN115973)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-TNF alpha anticorps

Specificité: This antibody detects TNF-alpha.

Purification: Affinity Chromatography employing immobilized Rat TNF-a matrix

Immunogène: Highly pure (>98%) recombinant Rat TNF-alpha

Information d'application

Indications d'application: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of Rat TNF-alpha(2.0 ng/mL), a concentration of 0.07 - 0.1 μg/mL of this antibody is required. ELISAIndirect: To detect Rat TNF-alpha by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant Rat TNF-alpha. Sandwich: To detect Rat TNF-alpha by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antibody, inconjunction with biotinylated Anti-Rat TNF-alpha (PP039B1 or PP039B2) as a detectionantibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-alpha. Western BlotTo detect Rat TNF-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Rat TNF-alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge the vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor (TNF-Alpha)

Sujet: TNF-alpha is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

ID gène: 24835

NCBI Accession: NP_036807

UniProt: P16599

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome