TNF alpha anticorps (Biotin)

N° du produit ABIN116241

Cet anticorps anti-TNF alpha Polyclonal Lapin conjugué à Biotin (ABIN116241) détecte spécifiquement TNF alpha dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNF alpha anticorps (Biotin) (ABIN116241)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNF alpha est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-TNF alpha anticorps (Biotin)

Purification: Affinity Chromatography

Immunogène: Highly pure (>98%) E.coli derived recombinant Human TNF-Alpha (Cat.-No PA132)

Information d'application

Indications d'application: ElisaDirect: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 -0.4 ng/well of recombinant hTNF-α. Sandwich: Using 100 μL/well antibody solution, a concentration of 0.25 - 1.0 μg/mL isrequired. In conjunction with polyclonal Anti-Human TNF-α as a capture antibody, it allowsthe detection of at least 0.2 - 0.4 ng/well of recombinant hTNF-α. Western Blot1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Centrifuge vial prior to opening.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody can be stored at -20 °C. Following reconstitution, store at 2-8 °C for up to two weeks or at -20 °C for up to six months.

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor (TNF-Alpha)

Sujet: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

ID gène: 9606

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome