Osteoprotegerin anticorps

N° du produit ABIN116322

Détail du produit anti-Osteoprotegerin anticorps

Antigène: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Osteoprotegerin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: Recognizes Human Osteoprotegerin (hOPG). Other species not tested.

Purification: Affinity Chromatography employing immobilized hOPG matrix

Immunogène: Highly purified (>98%) E. Coli derived recombinant Human OPG. Remarks: Molecular Weight: 150 kDa

Information d'application

Indications d'application: Sandwich ELISA: To detect Human OPG by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated anti-Human OPG antibody (PP1107B1 orPP1107B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human OPG. Western Blot To detect Human OPG by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human OPG is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: This antibody stained Formalin-Fixed Paraffin-Embedded Sectionsof Human breast invasive ductal carcinoma. Use at 0.5 μg/mL with overnight incubation at 4 °C. An HRP-labeled polymer detectionsystem was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0Sodium Citrate buffer is recommended.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Détails sur Osteoprotegerin

Antigène: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Autre désignation: Osteoprotegerin / TNFRSF11B (TNFRSF11B Produits)

Synonymes: anticorps CD254, anticorps ODF, anticorps OPGL, anticorps OPTB2, anticorps RANKL, anticorps TRANCE, anticorps hRANKL2, anticorps sOdf, anticorps OCIF, anticorps OPG, anticorps TR1, anticorps Opg, anticorps ocif, anticorps opg, anticorps tr1, anticorps TNF superfamily member 11, anticorps TNF receptor superfamily member 11b, anticorps tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin), anticorps TNF receptor superfamily member 11B, anticorps tumor necrosis factor receptor superfamily member 11b S homeolog, anticorps tumor necrosis factor receptor superfamily, member 11b, anticorps TNFSF11, anticorps TNFRSF11B, anticorps Tnfrsf11b, anticorps tnfrsf11b.S

Classe de substances: Chemical

Sujet: Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.Synonyms: OCIF, OPG, Osteoclastogenesis inhibitory factor, Tumor necrosis factor receptor superfamily member 11B

ID gène: 4982

NCBI Accession: NP_002537

UniProt: O00300