IFNB1 anticorps

N° du produit ABIN116507

L’anticorps Lapin Polyclonal anti-IFNB1 a été validé pour WB, IHC (p), EIA et Func. Il convient pour détecter IFNB1 dans des échantillons de Humain.

Aperçu rapide pour IFNB1 anticorps (ABIN116507)

Antigène: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IFNB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-IFNB1 anticorps

Specificité: This antibody detects Interferon-beta (IFN-beta).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Antigen Affinity Chromatography using immobilized IFN-beta matrix

Immunogène: Highly purified (>98%) E.coli derived recombinant Human Inferferon beta

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman IFN-beta (1.0 ng/mL), a concentration of 0.015-0.025 μg/mL of this antibody isrequired. Indirect ELISA: To detect Human IFN-beta by Indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IFNB1

Antigène: IFNB1 (Interferon, beta 1, Fibroblast (IFNB1))

Autre désignation: IFNB / Interferon beta

Sujet: The mammalian type I Inteferons (IFN1) are produced in response to viral infection and other inducers. They are divided into alpha and beta subtypes leukocytes and fibroblasts reactivity. The human IFN alphas are encoded by a family of at least 15 different genes, while IFN beta is the unique member of its subtype. There is approximately 50 % amino acid homology between the alpha and beta subtypes. Both IFN subtypes are pleiotropic cytokines and have a similar range of biological activities. Differences between alpha subtypes, and between IFN alpha and betas, are in potency and cell type specific activities. In particular, IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.Synonyms: Fibroblast interferon, IFB, IFN-beta, IFNB1

ID gène: 3456

NCBI Accession: NP_002167

UniProt: P01574

Pathways: Signalistation JAK/STAT, TCR Signaling, Signalisation TLR, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Autophagy, Inflammasome