NFYB anticorps (N-Term)

N° du produit ABIN116537

Détail du produit anti-NFYB anticorps

Antigène: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFYB est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Information d'application

Indications d'application: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NF-Y (B subunit specific) at a dilution of 1: 2,000 followed by reaction withPeroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (B subunit specific) is suitable for the detection by Immunoblot of human andmouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Yusing 0.5 to 1.0 mLper assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 90.0 mg/mL (by Refractometry)

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store original vial at 2-8 °C. After reconstitution store at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur NFYB

Antigène: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Autre désignation: NFYB (NFYB Produits)

Synonymes: anticorps CBF-A, anticorps CBF-B, anticorps HAP3, anticorps NF-YB, anticorps NFYB, anticorps nfyb, anticorps zgc:110533, anticorps zgc:110552, anticorps hap3, anticorps cbf-a, anticorps cbf-b, anticorps CBFB, anticorps Nfya, anticorps AA985999, anticorps Cbf-A, anticorps nuclear transcription factor Y subunit beta, anticorps nuclear transcription factor Y subunit beta L homeolog, anticorps nuclear transcription factor Y, beta b, anticorps nuclear transcription factor Y, beta a, anticorps nuclear transcription factor-Y beta, anticorps NFYB, anticorps nfyb.L, anticorps nfybb, anticorps nfyba, anticorps nfyb, anticorps Nfyb

Sujet: The Y box is a CCAAT box which is bound by the heteromeric DNA binding protein, NFY (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1 which also bind CCAAT-like sequences, NFY exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30 % of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: HAP3, Nuclear transcription factor Y subunit beta

ID gène: 4801

NCBI Accession: NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha