Ovalbumin anticorps

N° du produit ABIN116785

Détail du produit anti-Ovalbumin anticorps

Antigène: Ovalbumin (OVA)

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ovalbumin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.

Purification: Delipidation and Defibrination

Immunogène: Ovalbumin from Hen Egg White.

Information d'application

Indications d'application: Suitable for Immunoblotting (Western: 1/500-1/5000 or Dot blot), ELISA (1/5000-1/20000),Immunoprecipitation (1/100) and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Ovalbumin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(Cat#R1364HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1/90,000 to 1/450,000of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Ovalbumin

Antigène: Ovalbumin (OVA)

Autre désignation: Ovalbumin (OVA Produits)

Synonymes: anticorps 1600019A21Rik, anticorps R86, anticorps SPI-CI, anticorps Spi10, anticorps ovalbumin, anticorps NK13, anticorps Spi12, anticorps BC052216, anticorps NK21, anticorps NK21L1, anticorps Spi13, anticorps NK26, anticorps Spi14, anticorps AW540195, anticorps AT2, anticorps Spi9, anticorps EID, anticorps SPI3B, anticorps Gm11396, anticorps OTTMUSG00000000720, anticorps SERPINB14, anticorps C76171, anticorps C76174, anticorps SPI3C, anticorps SPIC, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6b, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9f, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9e, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 9d, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene, anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6e, anticorps ovalbumin (SERPINB14), anticorps serine (or cysteine) peptidase inhibitor, clade B, member 6c, anticorps Serpinb9b, anticorps Serpinb6b, anticorps Serpinb9f, anticorps Serpinb9e, anticorps Serpinb9d, anticorps Serpinb1-ps1, anticorps Serpinb6e, anticorps OVAL, anticorps Serpinb6c

Sujet: Chicken Ovalbumin is the major protein in the "white" of the egg (and a favorite antigen in immunological research). Egg white contains a variety of proteins including ovalbumin, conalbumin, ovomucoid and lysozyme. It belongs to the serpin family and the Ov serpin subfamily. Ovalbumin can cause an allergic reaction in humans. Ovalbumin has been implicated in the development of the egg shell. Immunohistochemistry revealed that ovalbumin is found only in the mammillary bodies of decalcified shell, and is not distributed throughout the shell matrix. These results indicate that ovalbumin is present during the initial phase of shell formation and becomes incorporated into the protein matrix of the mammillary bodies. However, it is not yet clear whether ovalbumin at this site plays a specific role in shell mineralisation. Anti Ovalbumin Antibody recognizes ovalbumin that is the main protein found in egg white. Ovalbumin makes up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily, but unlike most serpins it is not a serine protease inhibitor. The function of ovalbumin is unknown, although it is presumed to be a storage protein.Synonyms: Allergen Gal d II, Egg albumin, Plakalbumin, SERPINB14

ID gène: 396058

NCBI Accession: NP_990483

UniProt: P01012