Recombinant HMGB1 anticorps

N° du produit ABIN1169270

Détail du produit anti-HMGB1 anticorps

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: Recognizes human, mouse and rat HMGB1.

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Purified from HEK 293 cell culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Human recombinant HMGB1.

Clone: Giby-1-4

Isotype: IgG2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

HMGB1, monoclonal antibody (recombinant) (Giby1-4) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: HMGB1 (HMGB1 Produits)

Synonymes: anticorps HMG1, anticorps HMG3, anticorps SBP-1, anticorps DEF, anticorps HMG-1, anticorps Hmg1, anticorps amphoterin, anticorps p30, anticorps hmgb1, anticorps ik:tdsubc_1a5, anticorps wu:fb23c02, anticorps xx:tdsubc_1a5, anticorps zgc:56110, anticorps zgc:77104, anticorps hmg-1, anticorps hmg3, anticorps sbp-1, anticorps hmg1, anticorps HMGB1, anticorps Ac2-008, anticorps high mobility group box 1, anticorps high-mobility group box 1, anticorps high mobility group box 1a, anticorps high mobility group box 1 L homeolog, anticorps high mobility group protein B1, anticorps HMGB1, anticorps Hmgb1, anticorps hmgb1, anticorps hmgb1a, anticorps hmgb1.L, anticorps LOC100359149

Sujet: HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis. Anti-HMGB1, mAb (recombinant) (Giby-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P09429

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome