IL-8 anticorps (AA 23-101) (Biotin)

N° du produit ABIN1172239

Cet anticorps Lapin Polyclonal détecte spécifiquement IL-8 dans WB, IHC et ELISA. Il présente une réactivité envers Mouton.

Aperçu rapide pour IL-8 anticorps (AA 23-101) (Biotin) (ABIN1172239)

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-8 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-IL-8 anticorps (Biotin)

Épitope: AA 23-101

Séquence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AVLSRMST ELRCQCIKTH STPFHPKFIK ELRVIESGPH CENSEIIVKL TNGKEVCLDP KEKWVQKVVQ AFLKRAEKQD P

Specificité: It has been selected for its ability to recognize IL8 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant IL8 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IL8 conjugated to biotin.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant IL8 (Ala23~Pro101) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur IL-8

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy