HMGB1 anticorps

N° du produit ABIN1176834

Cet anticorps anti-HMGB1 est un anticorps Souris Monoclonal détectant HMGB1 dans WB, IHC, IF, Inhibition et CA. Adapté pour Mammifères. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour HMGB1 anticorps (ABIN1176834)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)

Clone: DPH1-1

Détail du produit anti-HMGB1 anticorps

Immunogène: 17-mer peptide KGKPDAAKKGVVKAEKS

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Conseil sur la manipulation: DPH1.1 mAb is provided freeze-dried.

Stock: -20 °C

Références pour anticorps anti-HMGB1 (ABIN1176834)

Han, Yuan, Deng, He, Zhang, Shen, Chen, Qian: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." dans: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome