HMGB1 anticorps

N° du produit ABIN1176834

Cité dans 1 publication. Cet anticorps anti-HMGB1 Monoclonal Souris (Clone DPH1-1) (ABIN1176834) détecte spécifiquement HMGB1 dans WB, IHC, IF, Inhibition et CA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Mammifères.

Aperçu rapide pour HMGB1 anticorps (ABIN1176834)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Humain, Souris, Rat, Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)

Clone: DPH1-1

Détail du produit anti-HMGB1 anticorps

Fonction: anti HMGB1 monoclonal antibody

Séquence: KGKPDAAKKGVVKAEKS

Réactivité croisée (Details): DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2.

Attributs du produit: DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Immunogène: The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide P1 (KGKPDAAKKGVVKAEKS). Hybridomas were generated from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.

Commentaires:

DPH1.1 mAb can be used for Western blot (WB).
DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays.
In vivo, DPH1.1 mAb administered intravenously (220 μg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized protein once reconstituted will be dissolved in a solution containing Na3PO4 50mM and NaCl 0.15M pH 7.4.

Buffer: The antibody is lyophilized from Na3PO4 50mM and NaCl 0.15M pH 7.4

Stock: -20 °C

Stockage commentaire: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C), thawed and re-frozen.

Références pour anticorps anti-HMGB1 (ABIN1176834)

Han, Yuan, Deng, He, Zhang, Shen, Chen, Qian: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." dans: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High-mobility group protein B1 (HMGB1)

Sujet: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome