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MEK1 anticorps (pSer218) (Alexa Fluor 488)

MAP2K1 Reactivité: Humain, Souris ICS Hôte: Souris Monoclonal O24 Alexa Fluor 488
N° du produit ABIN1177096
  • Antigène Voir toutes MEK1 (MAP2K1) Anticorps
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Épitope
    • 46
    • 28
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    pSer218
    Reactivité
    • 375
    • 205
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    Humain, Souris
    Hôte
    • 340
    • 32
    • 3
    • 2
    • 2
    Souris
    Clonalité
    • 293
    • 86
    Monoclonal
    Conjugué
    • 181
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    Cet anticorp MEK1 est conjugé à/à la Alexa Fluor 488
    Application
    • 247
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    Intracellular Staining (ICS)
    Marque
    BD Phosflow™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Phosphorylated Human MEK1 Peptide
    Clone
    O24
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MAP2K1 Anticorps primaire
  • Volume d'échantillon
    5 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
  • Frémin, Meloche: "From basic research to clinical development of MEK1/2 inhibitors for cancer therapy." dans: Journal of hematology & oncology, Vol. 3, pp. 8, (2010) (PubMed).

    Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." dans: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, (2006) (PubMed).

    Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." dans: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, (2001) (PubMed).

    Papin, Eychène, Brunet, Pagès, Pouysségur, Calothy, Barnier: "B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222." dans: Oncogene, Vol. 10, Issue 8, pp. 1647-51, (1995) (PubMed).

    Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." dans: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, (1992) (PubMed).

  • Antigène
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Autre désignation
    MEK1 , MEK (MAP2K1 Produits)
    Synonymes
    anticorps Mek1, anticorps CFC3, anticorps MAPKK1, anticorps MEK1, anticorps MKK1, anticorps PRKMK1, anticorps MEKK1, anticorps Prkmk1, anticorps mek-2, anticorps si:ch211-242m18.2, anticorps wu:fj56a12, anticorps wu:fj61b01, anticorps zgc:56557, anticorps ATMEK1, anticorps F20B18.180, anticorps F20B18_180, anticorps MAP kinase/ ERK kinase 1, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE, anticorps MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, anticorps NMAPKK, anticorps MEK, anticorps mitogen activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1, anticorps mitogen-activated protein kinase kinase 1 L homeolog, anticorps MAP kinase/ ERK kinase 1, anticorps Map2k1, anticorps MAP2K1, anticorps map2k1.L, anticorps map2k1, anticorps MEK1
    Sujet
    The O24-836 monoclonal antibody specifically binds to the human MEK1 pS218 and MEK2 pS222 phosphorylated sites. MEK1 and MEK2 (MEK1/MEK2) are ~45 kDa dual-specificity protein kinases that are also known as MAPK/ERK Kinase 1 and 2 and MAP kinase kinases (aka, MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). MEK1 and MEK2 activation is dependent upon phosphorylation of certain amino acid residues including serine 218 (pS218) of MEK1 and serine 222 (pS222) of MEK2 by MAP kinase kinase kinases (MAP3Ks), such as by Raf kinases. MEK1/MEK2 can phosphorylate and thus activate the MAP (Mitogen-Activated Protein) kinases, ERK1 and ERK2. The ERK kinases are critically involved in multiple signal transduction pathways induced by growth factors, cytokines, and mitogens. These kinases regulate cellular growth, differentiation, survival, motility and angiogenesis. Dysregulated activation of MEK1/2 is observed in tumor cells. During antibody development, the purified O24-836 monoclonal antibody was found to detect phosphorylated MEK1/MEK2 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. This antibody crossreacts with phosphorylated MEK1/MEK2 expressed by mouse cells as tested by Western blot analysis and immunofluorescent staining and flow cytometric analysis.
    Synonyms: MAP2K1/2, MAPKK1/2, MAP kinase kinase 1/2, MAP2K1/2, PRKMK1/2
    Pathways
    Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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