TNF alpha anticorps

N° du produit ABIN1177286

Il existe 3+ publications pour ce produit. L’anticorps anti-TNF alpha Monoclonal Souris est utilisé pour la détection de TNF alpha dans des échantillons de Humain. Il a été validé pour ELISA et Neut.

Aperçu rapide pour TNF alpha anticorps (ABIN1177286)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: ELISA, Neutralization (Neut)

Clone: MABTNF-A5

Détail du produit anti-TNF alpha anticorps

Attributs du produit: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human TNF. Neutralization activity may be measured using a cytolysis assay for MTT dye conversion with 2.0 ng/mL recombinant human TNF (Cat. No. 554618) and L929 at 2 x 10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human TNF for 60 minutes).
50% Neutralization (ND50) at 1 -100 ng/ml
>90% Neutralization at 50-300 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Recombinant human TNF protein

Isotype: IgG2a kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-TNF alpha (ABIN1177286)

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." dans: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).

Kriegler, Perez, DeFay, Albert, Lu: "A novel form of TNF/cachectin is a cell surface cytotoxic transmembrane protein: ramifications for the complex physiology of TNF." dans: Cell, Vol. 53, Issue 1, pp. 45-53, (1988) (PubMed).

Smith, Baglioni: "The active form of tumor necrosis factor is a trimer." dans: The Journal of biological chemistry, Vol. 262, Issue 15, pp. 6951-4, (1987) (PubMed).

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet: The MABTNF-A5 antibody, formerly known as 5N, reacts with human tumor necrosis factor, TNF (aka, TNF-alpha and TNFSF2). TNF is a multifunctional cytokine involved in a variety of immune and inflammatory responses including hemorrhagic necrosis of tumors, septic shock, fever and autoimmune diseases. TNF can regulate the growth and differentiation of many different cell types. It is produced by different activated cell types including macrophages, T lymphocytes, NK cells, dendritic cells, endothelial cells, peripheral blood leukocytes, osteoblasts, astrocytes, mast cells, Kupffer cells, smooth muscle cells, fibroblasts and certain tumor cells. TNF exists in two biologically active forms, i.e., transmembrane and soluble forms. Upon activation, cells express transmembrane TNF glycoproteins that associate as homotrimeric complexes. After enzymatic cleavage, the extracellular region of membrane TNF sheds as a soluble homotrimer. The biologically active form of TNF has been reported to be a trimer. The immunogen used to generate the MABTNF-A5 hybridoma was purified recombinant human TNF. This is an extraordinarily potent neutralizing antibody that is reportedly also useful for ELISA measurements of human TNF.

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome