PLG anticorps

N° du produit ABIN118044

Cité dans 3 publications. Cet anticorps anti-PLG Polyclonal Chèvre (ABIN118044) détecte spécifiquement PLG dans WB, IP et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PLG anticorps (ABIN118044)

Antigène: PLG (Plasminogen (PLG))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PLG est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-PLG anticorps

Specificité: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Plasminogen (Human Plasma). Cross reactivity against Plasminogen from other sources is unknown.

Purification: Delipidation and defibrination

Immunogène: Plasminogen (Human Plasma)

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Plasminogen [Human Plasma] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 With 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -20 °C or below.

Références pour anticorps anti-PLG (ABIN118044)

Funk, Schaarschmidt, Slesiona, Hallström, Horn, Brock: "The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species." dans: International journal of medical microbiology : IJMM, Vol. 306, Issue 1, pp. 59-68, (2016) (PubMed).

Koenigs, Stahl, Averhoff, Göttig, Wichelhaus, Wallich, Zipfel, Kraiczy: "CipA of Acinetobacter baumannii Is a Novel Plasminogen Binding and Complement Inhibitory Protein." dans: The Journal of infectious diseases, Vol. 213, Issue 9, pp. 1388-99, (2016) (PubMed).

Schott, Grosskinsky, Brenner, Kraiczy, Wallich: "Molecular characterization of the interaction of Borrelia parkeri and Borrelia turicatae with human complement regulators." dans: Infection and immunity, Vol. 78, Issue 5, pp. 2199-208, (2010) (PubMed).

Détails sur PLG

Antigène: PLG (Plasminogen (PLG))

Autre désignation: Plasminogen / PLG

ID gène: 5340

NCBI Accession: NP_000292

UniProt: P00747

Pathways: Système du Complément, Lipid Metabolism