TNF alpha anticorps

N° du produit ABIN118714

Cet anticorps Rat Monoclonal détecte spécifiquement TNF alpha dans WB, FACS, EIA et IHC (fro). Il présente une réactivité envers Souris.

Aperçu rapide pour TNF alpha anticorps (ABIN118714)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TNF alpha est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MP6-XT3

Détail du produit anti-TNF alpha anticorps

Purification: Affinity chromatography on Protein G

Immunogène: Recombinant murine TNF-alpha expressed in E. coli.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot. ELISA: 5 μg/mL. Flow Cytometry: Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur TNF alpha

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor (TNF-Alpha)

Sujet: This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumour cell lines, and has an important immunoregulatory role.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

ID gène: 10090

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome