CD56 anticorps (Biotin)

N° du produit ABIN118741

Détail du produit anti-CD56 anticorps (Biotin)

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivité: Humain, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD56 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Specificité: Recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM) (1).

Immunogène: Human retinoblastoma tumor tissue, membrane fraction

Clone: ERIC-1

Isotype: IgG1

Information d'application

Indications d'application: Studies of neural cell adhesion molecule. May also be used to study isoforms of NCAM expressed on different tumor types. Western blot for analysis of isoforms expressed, did not react with any of the leukemias or lymphomas tested (1). Flow cytometric applications (1 μg to stain 6 x 10e5 cells). Not for use in IHC with formalin-fixed, paraffin embedded sections. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20 °C) for 5 minutes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 100 µg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.2, containing 2.0 mM EDTA, 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD56

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Autre désignation: CD56 (NCAM1 Produits)

Synonymes: anticorps CD56, anticorps MSK39, anticorps NCAM, anticorps fj56g09, anticorps ncam, anticorps ncam1, anticorps wu:fj56g09, anticorps NCAM1, anticorps n-cam, anticorps E-NCAM, anticorps NCAM-1, anticorps Ncam, anticorps Cd56, anticorps N-CAM, anticorps N-CAM-1, anticorps NCAM-C, anticorps NCAMC, anticorps N-CAM-1-B, anticorps NCAM-1-B, anticorps ncam1-a, anticorps ncam1-b, anticorps neural cell adhesion molecule 1, anticorps neural cell adhesion molecule 1a, anticorps neural cell adhesion molecule 1 S homeolog, anticorps neural cell adhesion molecule 1 L homeolog, anticorps NCAM1, anticorps ncam1a, anticorps ncam1, anticorps LOC100085373, anticorps ncam1.S, anticorps Ncam1, anticorps ncam1.L

Sujet: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.Synonyms: N-CAM-1, NCAM, NCAM-1, Neural cell adhesion molecule 1

ID gène: 4684

NCBI Accession: NP_000606

UniProt: P13591