GZMB anticorps

N° du produit ABIN119563

Détail du produit anti-GZMB anticorps

Antigène: GZMB (Granzyme B (GZMB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GZMB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody recognises the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Recombinant Granzyme B. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Clone: GB7

Isotype: IgG2a

Information d'application

Indications d'application: Immunohistochemistry on Paraffin Sections (1/50-1/200): Requires antigen retrieval using heat treatment with sodium citrate buffer pH 6.0. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: TRIS buffered saline, pH 8.0, 0.01 % Thiomersal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur GZMB

Antigène: GZMB (Granzyme B (GZMB))

Abstract: GZMB Produits

Synonymes: anticorps CCPI, anticorps CGL-1, anticorps CGL1, anticorps CSP-B, anticorps CSPB, anticorps CTLA1, anticorps CTSGL1, anticorps HLP, anticorps SECT, anticorps granzyme B, anticorps GZMB

Sujet: Granzyme A and Granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.Synonyms: CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Cathepsin G-like 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Granzyme-2, Lymphocyte protease, SECT, T-cell serine protease 1-3E

ID gène: 3002

NCBI Accession: NP_004122

UniProt: P10144

Pathways: Apoptose, Caspase Cascade in Apoptosis