Neisseria Meningitidis Antigen Orf1 anticorps
Reactivité: Neisseria meningitidis
WB
Hôte: Lapin
Polyclonal
unconjugated
1 image
(1 reference)-
- Antigène
- Neisseria Meningitidis Antigen Orf1
- Reactivité
- Neisseria meningitidis
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Inconjugué
- Application
- Western Blotting (WB)
- Specificité
- The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
- Réactivité croisée (Details)
- Neisseria meningitidis
- Purification
- Purified by ligand affinity chromatography.
- Pureté
- > 95 % (by SDS-PAGE)
- Immunogène
- Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
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- Indications d'application
- Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Do not freeze.
- Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Do not freeze.
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: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." dans: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
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: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." dans: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
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- Antigène
- Neisseria Meningitidis Antigen Orf1
- Autre désignation
- Neisseria meningitidis antigen Orf1 / FrpD
- Sujet
- Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD
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