EGFR anticorps (pTyr869) (Biotin)

N° du produit ABIN126759

Cet anticorps anti-EGFR Monoclonal Souris conjugué à Biotin (Clone 12A3) (ABIN126759) détecte spécifiquement EGFR dans WB, IF, IHC (p), IP, EIA et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour EGFR anticorps (pTyr869) (Biotin) (ABIN126759)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 12A3

Détail du produit anti-EGFR anticorps (Biotin)

Épitope: pTyr869

Specificité: This antibody specifically recognizes EGFR phosphorylated at Tyrosine 869 and detects EGFR activation after interaction with src kinases. Mab 12A3 does not crossreact with the highly homologues pTyr 877 of activated erbB2.

Attributs du produit: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Size exclusion chromatography

Ingrédients: incl. positive Control

Immunogène: Phosphopeptide conjugated to KLH.

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA). Immunoprecipitation: 1 - 10 μg per 106 vanadate treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL. Immunohistochemistry on frozen and paraffin sections. Luminex.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-80 °C

Stockage commentaire: Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR / ERBB1

Sujet: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins