EGFR anticorps (pTyr1172)

N° du produit ABIN126775

Cet anticorps Souris Monoclonal détecte spécifiquement EGFR dans WB. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour EGFR anticorps (pTyr1172) (ABIN126775)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB)

Clone: 10G12

Détail du produit anti-EGFR anticorps

Épitope: pTyr1172

Specificité: This antibody specifically recognizes EGFR phosphorylated at Tyrosine 1172.

Attributs du produit: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent ultrafiltration and size exclusion

Ingrédients: incl. positive Control

Immunogène: Phosphopeptide conjugated to hemocyaninN P D pY Q Q D

Isotype: IgG

Information d'application

Indications d'application: Western blot (0.5 μg/mL for HRPO/ECL detection, recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer). Use cell lysate from EGF-treated HepG2 cells as positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocole: Positive control cell lysate provided: HepG2 EGF Treated - recommended for immunoblottingFormulation: Lyophilized cell lysate from HepG2 cells. Serum starved cells were treated for 15 min. withEGF. Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGEsample buffer, mix and incubate at 90°C for 5 min. Store in aliquots at -20°C. Avoidrepeated freezing and thawing. Applications: 20 µl of positive cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) forHRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysate contains SDS and is not recommended for applications withnative proteins such as immunoprecipitation.

Restrictions: For Research Use only

Stockage

Reconstitution: Reconstitute with 1 mL H2O (15 min, RT).

Buffer: PBS, 0.09 % Na-azide, PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-EGFR (ABIN126775)

Song, Yu, Sun, Feng, Yu, Khan, Zhu, Huang, Li, Mok, Cheng, Gao, Feng: "Androgen receptor drives hepatocellular carcinogenesis by activating enhancer of zeste homolog 2-mediated Wnt/β-catenin signaling." dans: EBioMedicine, Vol. 35, pp. 155-166, (2019) (PubMed).

Yu, Feng, Sun, Zhuo, Li, Zhou, Huang, Jiang, Zhu, Zhang, Le, Zheng, Cheng, Gao: "Bufalin suppresses hepatocarcinogenesis by targeting β-catenin/TCF signaling via cell cycle-related kinase." dans: Scientific reports, Vol. 8, Issue 1, pp. 3891, (2018) (PubMed).

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR / ERBB1

Sujet: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins