EGFR anticorps (Tyr1197)

N° du produit ABIN126780

Cet anticorps anti-EGFR Monoclonal Souris (Clone 20G3) (ABIN126780) détecte spécifiquement EGFR dans WB, IF, IP et EIA. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour EGFR anticorps (Tyr1197) (ABIN126780)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 20G3

Détail du produit anti-EGFR anticorps

Épitope: Tyr1197

Specificité: This antibody specifically recognizes non-activated EGF-receptor (dephosphorylated at Y1197) and is interacting with the 1197 - N A E Y L R V peptide motif. It does not interact with the activated EGF-receptor phosphorylated at Y1197.

Attributs du produit: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Purification: Subsequent thiophilic adsorption and size exclusion chromatography

Ingrédients: incl. positive Control

Immunogène: Peptide conjugated to KLHRemarks: Epitope: dephospho-Y1173

Isotype: IgG1

Information d'application

Indications d'application: ELISA (0.05 μg/mL). Western blot. Immunoprecipitation (1 - 10 μg per 10^6 vanadate treated A431 cells). Immunoflourescence (1-10 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: PBS, 0.09 % Na-azide, PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store lyophilised product upon arrival at -20 °C. Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze inliquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate, we recommend centrifugation before use.
Shelf life: One year from despatch.

Date de péremption: 12 months

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR / ERBB1

Sujet: EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y869 by src family kinases. Phosphorylation of Y1069 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1071 by CamKinase II leads to attenuation of kinase activity, phosphorylation of T678 (by PKC) and T693 (by MAPK, p38) interferes with receptor endocytosis/recycling.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

ID gène: 1956

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins