HSP27 anticorps (pSer82)
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- Antigène Voir toutes HSP27 (HSPB1) Anticorps
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Épitope
- pSer82
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Reactivité
- Humain, Rat, Souris, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HSP27 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificité
- This antibody specifically interacts with HSP27 phosphorylated at Serine 82 and does not crossreact with the non-phosphorylated HSP27 nor with unrelated Serine-phosphorylated proteins.
- Attributs du produit
- Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
- Purification
- Size Exclusion Chromatography
- Ingrédients
- incl. positive Control
- Immunogène
- Synthetic phosphopeptide conjugated to KLH.
- Clone
- 5B9
- Isotype
- IgG2a
- Top Product
- Discover our top product HSPB1 Anticorps primaire
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- Indications d'application
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from EGF-treated HepG2 cellsImmunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mLPBS containing 0.09 % Sodium Azide, PEG and Sucrose
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Autre désignation
- HSPB1 / HSP27 (HSPB1 Produits)
- Synonymes
- anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L
- Sujet
- The small heat shock protein hsp27 is constitutively expressed in most cell lines. The expression level is increased in response to environmental stress. Activation of the p38 kinases results in subsequent activation of MAPKAP2 that phosphorylates hsp27 at Ser15, Ser78 and Ser82.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
- ID gène
- 3315
- UniProt
- P04792
- Pathways
- Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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