ERK2 anticorps (C-Term)
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- Antigène Voir toutes ERK2 (MAPK1) Anticorps
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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Épitope
- C-Term
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Reactivité
- Humain, Souris, Rat, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp ERK2 est non-conjugé
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- Specificité
- This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2). It does not crossreact with MAP kinase 1 (ERK1).
- Attributs du produit
- Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
- Purification
- Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
- Ingrédients
- incl. positive Control
- Immunogène
- Peptide conjugated to KLHEpitope: C-terminus
- Clone
- 6G11
- Isotype
- IgG1
- Top Product
- Discover our top product MAPK1 Anticorps primaire
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- Indications d'application
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ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: 1-10 μg/mL per 10^6 pervanadate-treated A431 cells. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL H2O (15 min, RT).
- Buffer
- 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
- Autre désignation
- ERK2 (MAPK1 Produits)
- Synonymes
- anticorps ERK-1, anticorps ERK1, anticorps ERT2, anticorps HS44KDAP, anticorps HUMKER1A, anticorps P44ERK1, anticorps P44MAPK, anticorps PRKM3, anticorps p44-ERK1, anticorps p44-MAPK, anticorps Erk-1, anticorps Erk1, anticorps Ert2, anticorps Esrk1, anticorps Mnk1, anticorps Mtap2k, anticorps Prkm3, anticorps p44, anticorps p44erk1, anticorps p44mapk, anticorps ERK, anticorps ERK2, anticorps ERT1, anticorps MAPK2, anticorps P42MAPK, anticorps PRKM1, anticorps PRKM2, anticorps p38, anticorps p40, anticorps p41, anticorps p41mapk, anticorps 9030612K14Rik, anticorps AA407128, anticorps AU018647, anticorps C78273, anticorps Erk2, anticorps Prkm1, anticorps p42mapk, anticorps zERK2, anticorps MAPK1, anticorps Xp42, anticorps erk, anticorps erk2, anticorps ert1, anticorps mapk, anticorps mapk1b, anticorps mapk2, anticorps mpk1, anticorps prkm1, anticorps prkm2, anticorps mapk1, anticorps mapk1a, anticorps xp42, anticorps ATMPK1, anticorps F14N23.9, anticorps F14N23_9, anticorps MITOGEN-ACTIVATED PROTEIN KINASE, anticorps mitogen-activated protein kinase 1, anticorps MNK1, anticorps ATMPK2, anticorps MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2, anticorps T30E16.13, anticorps T30E16_13, anticorps mitogen-activated protein kinase homolog 2, anticorps mitogen-activated protein kinase 3, anticorps mitogen-activated protein kinase 1, anticorps mitogen activated protein kinase 1, anticorps mitogen-activated protein kinase 1 L homeolog, anticorps mitogen-activated protein kinase 1 S homeolog, anticorps mitogen activated protein kinase 3, anticorps mitogen-activated protein kinase homolog 2, anticorps MAPK3, anticorps Mapk3, anticorps MAPK1, anticorps Mapk1, anticorps mapk1, anticorps mapk1.L, anticorps mapk1.S, anticorps PfMAP1, anticorps MPK1, anticorps PVX_084965, anticorps LOC9328617, anticorps Tsp_01601, anticorps MPK2
- Sujet
- Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
- Poids moléculaire
- 42 kDa
- ID gène
- 5594
- UniProt
- P28482
- Pathways
- Signalisation MAPK, Signalisation RTK, Apoptose, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
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