MEK1 anticorps

N° du produit ABIN126837

Cet anticorps anti-MEK1 Monoclonal Souris (Clone 9G3) (ABIN126837) détecte spécifiquement MEK1 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat et Chien.

Aperçu rapide pour MEK1 anticorps (ABIN126837)

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MEK1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 9G3

Détail du produit anti-MEK1 anticorps

Specificité: This antibody specifically recognizes the activation loop of MEK1/2 independent of its phosphorylation status.

Attributs du produit: Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAPKK1, MEK1, MKK1, PRKMK1,MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK2, MEK2, MKK2, PRKMK2

Purification: Size exclusion chromatography

Ingrédients: incl. positive Control

Immunogène: Synthetic peptide conjugated to KLH

Isotype: IgG2a

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated HepG2 cells. ELISA: 0.1 μg/mL (protein ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1 mL H2O (15 min, RT)

Buffer: 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur MEK1

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: MAP2K1

Sujet: MEK (MAP Kinase Kinase) phosphorylates the MAP Kinase on both threonine and tyrosine residues of the activation motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.Synonyms: MAP2K1 - mitogen-activated protein kinase kinase 1, MAP2K2 - mitogen-activated protein kinase kinase 2, MAPKK1, MAPKK2, MEK1, MEK2, MKK1, MKK2, PRKMK1, PRKMK2

ID gène: 5604

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling