HSP27 anticorps
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- Antigène Voir toutes HSP27 (HSPB1) Anticorps
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Reactivité
- Humain, Singe
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp HSP27 est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- Specificité
- The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots.
- Purification
- Purified
- Top Product
- Discover our top product HSPB1 Anticorps primaire
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- Indications d'application
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Western Blot: 1-2 μg/mL. Immunoprecipitation: 5-10 μg/mL. Imunocytochemistry: 10-15 μg/mL. ELISA: 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
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- Antigène
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Autre désignation
- HSPB1 / HSP27 (HSPB1 Produits)
- Synonymes
- anticorps CMT2F, anticorps HMN2B, anticorps HS.76067, anticorps HSP27, anticorps HSP28, anticorps Hsp25, anticorps SRP27, anticorps 27kDa, anticorps Hsp27, anticorps cb153, anticorps cb660, anticorps hsp1, anticorps hsp25, anticorps hsp27, anticorps id:ibd2821, anticorps sb:cb660, anticorps zgc:103437, anticorps HSPB1, anticorps cmt2f, anticorps hs.76067, anticorps hsp28, anticorps heat shock protein family B (small) member 1, anticorps heat shock protein 1, anticorps heat shock protein, alpha-crystallin-related, 1, anticorps heat shock protein family B (small) member 1 L homeolog, anticorps HSPB1, anticorps Hspb1, anticorps hspb1, anticorps hspb1.L
- Sujet
- Human Hsp27, mouse Hsp25 and αβ-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
- ID gène
- 3315
- NCBI Accession
- NP_001531
- UniProt
- P04792
- Pathways
- Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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