Caspase 9 anticorps (Asp330, N-Term)

N° du produit ABIN127028

L’anticorps anti-Caspase 9 Polyclonal Lapin est utilisé pour la détection de Caspase 9 dans des échantillons de Humain. Il a été validé pour WB, IF, IP et IHC (p).

Aperçu rapide pour Caspase 9 anticorps (Asp330, N-Term) (ABIN127028)

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 9 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Caspase 9 anticorps

Épitope: Asp330, N-Term

Specificité: The affinity purified antibody recognizing the active forms of caspase-9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. The anti-active caspase-9 antibody recognizes only the cleaved caspase-9 (37 kDa). It does not recognize full-length caspase-9 or any other caspases.

Purification: Affinity chromatography

Immunogène: Synthetic peptide mapping to the N-terminus adjacent to Asp330 of human Caspase-9

Information d'application

Indications d'application: Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunohistochemistry /Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS containing 50 % glycerol, 0.5 % BSA, and 0.02 % thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C to -70 °C.

Détails sur Caspase 9

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Autre désignation: Caspase-9

Sujet: Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase-9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with pro-caspase-9 (47 kDa) and Apaf-1. The complex processes pro-caspase-9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved Caspase-9 further processes other caspases including caspase-3 and caspase-6, to initiate a caspase cascade leading to apoptosis.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6

ID gène: 842

NCBI Accession: NP_001220

UniProt: P55211

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity