Caspase 9 anticorps

N° du produit ABIN127091

L’anticorps anti-Caspase 9 Polyclonal Lapin est utilisé pour la détection de Caspase 9 dans des échantillons de Souris et Rat. Il a été validé pour WB.

Aperçu rapide pour Caspase 9 anticorps (ABIN127091)

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 9 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-Caspase 9 anticorps

Specificité: The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved Caspase-9.

Purification: Protein A Chromatography.

Immunogène: Synthetic peptide surrounding amino acid 345 of Mouse Caspase-9

Information d'application

Indications d'application: Western Blot: 0.5-4.0 μg/mL. Mouse small intestine and rat kidney tissue lysates can be used as Positive Controls.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage.

Détails sur Caspase 9

Antigène: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Autre désignation: Caspase-9

Sujet: Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase- 9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6

ID gène: 842

NCBI Accession: NP_001220

UniProt: P55211

Pathways: Signalisation MAPK, Signalisation RTK, Apoptose, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity