PCSK2 anticorps (AA 95-107)

N° du produit ABIN129594

Détail du produit anti-PCSK2 anticorps

Antigène: PCSK2 (Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2))

Épitope: AA 95-107

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCSK2 est non-conjugé

Application: Western Blotting (WB), ELISA

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Immunogène: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.

Isotype: IgG

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in immunofluorescence staining of cultured cells and western blotting using transfected cell lysates.  The antibody is also functional by ELISA.  Reactivity in other immunoassays is unknown.  Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure below).  It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.36 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur PCSK2

Antigène: PCSK2 (Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2))

Autre désignation: PC2 (PCSK2 Produits)

Synonymes: anticorps NEC 2, anticorps NEC-2, anticorps NEC2, anticorps PC2, anticorps SPC2, anticorps PCSK2, anticorps 6330411F23Rik, anticorps AI839700, anticorps Nec-2, anticorps Nec2, anticorps Phpp-2, anticorps pc2, anticorps nec2, anticorps spc2, anticorps pcsk2, anticorps pcsk2-A, anticorps proprotein convertase subtilisin/kexin type 2, anticorps proprotein convertase subtilisin/kexin type 2 L homeolog, anticorps PCSK2, anticorps pcsk2, anticorps Pcsk2, anticorps pcsk2.L

Sujet: PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation.  Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
Synonyms: E3 SUMO-protein ligase CBX4 Chromobox protein homolog 4 Polycomb 2 homolog Pc2 hPc2

ID gène: 8535

UniProt: O00257

Pathways: Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity