EGFR anticorps (AA 1175-1200)

N° du produit ABIN129666

N° du produit (Fournisseur): 600-401-905

Cet anticorps anti-EGFR est un anticorps Lapin Polyclonal détectant EGFR dans WB, ELISA et IHC. Adapté pour Humain. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour EGFR anticorps (AA 1175-1200) (ABIN129666)

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EGFR est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-EGFR anticorps

Épitope: AA 1175-1200

N° du produit (Fournisseur): 600-401-905

Fournisseur: Rockland

Fonction: EGFR Antibody

Réactivité croisée (Details): EGFR Antibody is directed against human epidermal growth factor receptor (EGFR) and is useful in determining its presence in ELISA and western blotting experiments. This antibody can detect EGFR from human, mouse and rat sources.

Attributs du produit: Synonyms: rabbit anti-EGFR antibody, Epidermal growth factor receptor antibody, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erbb 1, Erbb-1, Erbb1, HER1 antibody

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus near amino acids 1175-1200 of human EGFR protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 170 kDa in size corresponding to EGFR protein by western blotting in the appropriate cell lysate or extract.   This antibody detects both non-phosphorylated and phosphorylated EGFR at residue Y1197.

Western Blot Dilution: 1:500 - 1:5,000

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Anti-EGFR at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-EGFR (ABIN129666)

Dong, Saunders, Silasi-Mansat, Yu, Zhu, Lupu, Towner, Dong, Chen: "Therapeutic efficacy of a synthetic epsin mimetic peptide in glioma tumor model: uncovering multiple mechanisms beyond the VEGF-associated tumor angiogenesis." dans: Journal of neuro-oncology, Vol. 138, Issue 1, pp. 17-27, (2019) (PubMed).

Détails sur EGFR

Antigène: EGFR (Epidermal Growth Factor Receptor (EGFR))

Autre désignation: EGFR

Sujet: Background: Anti-EGFR Antibody is ideal for Western Blotting, ELISA and IHC. EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development.  Growth factor receptors are involved not only in promoting the proliferation of normal cells but also in the aberrant growth of many types of human tumors.  For example, the epidermal growth factor receptor (EGFR) is mutated and/or overexpressed in many common solid human squamous cell carcinomas including breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. Over-expression of the EGFR gene occurs in carcinomas with and without gene amplification. EGFR and erbB-2 are particularly important in breast cancer because increased production or activation has been associated with poor prognosis.  EGFR belongs to a family of growth factor receptors, which also includes ErbB-2/HER-2/neu, ErbB-3/HER-3/neu and ErbB-4/HER-4neu. EGFR can heterodimerize with each of the members of this family.

ID gène: 1956, 29725609

UniProt: P00533

Pathways: Signalisation NF-kappaB, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins