RFC1 anticorps (AA 401-500) (Alexa Fluor 350)

N° du produit ABIN1390473

Détail du produit anti-RFC1 anticorps (Alexa Fluor 350)

Antigène: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Épitope: AA 401-500

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFC1 est conjugé à/à la Alexa Fluor 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Réactivité croisée: Humain, Souris

Homologie: Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human RFC1

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur RFC1

Antigène: RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))

Autre désignation: RFC1 (RFC1 Produits)

Synonymes: anticorps A1, anticorps MHCBFB, anticorps PO-GA, anticorps RECC1, anticorps RFC, anticorps RFC140, anticorps AtRFC1, anticorps replication factor C 1, anticorps replication factor C1, anticorps 140kDa, anticorps Alp145, anticorps Recc1, anticorps Replication factor C1, anticorps si:dkey-170e5.1, anticorps zgc:162875, anticorps replication factor C subunit 1, anticorps replication factor C1, anticorps replication factor C (activator 1) 1, anticorps DNA replication factor C complex subunit Rfc1, anticorps RFC1, anticorps Rfc1, anticorps rfc1

Sujet:

Synonyms: DNA binding Protein PO GA, DNA-binding protein PO-GA, MHC binding factor beta, MHCBFB, RECC1, Replication factor C 140 kDa subunit, Replication factor C, Replication factor C large subunit, Replication factor C subunit 1, Replication factor C1, RF-C 140 kDa subunit, RFC1, RFC1_HUMAN, RFC140, RFC140 Replication Factor C 140 kDa subunit, A1 140 kDa subunit, A1 P145 Activator 1 large subunit, Activator 1 140 kDa subunit, Activator 1 large subunit, Activator 1 subunit 1.

Background: Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (including RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC1 is a substrate for caspase-3 in vitro and is cleaved by a caspase-3-like protease during FAS-mediated apoptosis. In addition, phosphorylation of the PCNA binding domain of RFC1 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibits DNA synthesis. The human RFC1 gene maps to chromosome 4p14 and encodes the RFC1 subunit.

ID gène: 5981

UniProt: P35251

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport