Ammonium sulfate precipitation and affinity chromatography on protein A agarose
Immunogène
Recombinant E. coli RecA protein
Clone
ARM193
Isotype
IgG2b
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Reconstitution
Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water.
Concentration
1.0 mg/mL (following reconstitution)
Buffer
PBS ( pH 7.2), 0.09 % NaN3, 1 % sucrose
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted in aliquots at -20 °C.
Antigène
Recombinase A / RecA
Sujet
RecA protein (38 kDa) is important in various cellular functions such as DNA repair, genetic recombination, induction of prophages, mutagenesis, cell division, and DNA replication in E. coli. RecA has DNA-dependent ATPase activity, and promotes ATP-dependent pairing of double stranded DNA and homologous single stranded fragments to form D-loops.Synonyms: lexB, recH, rnmB, tif, umuB, zab