COL2A1 anticorps (Chain alpha 1)

N° du produit ABIN1450180

Détail du produit anti-COL2A1 anticorps

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Épitope: Chain alpha 1

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp COL2A1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificité: This product has been prepared by Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Crossreactivity < 1 % against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Immunoaffinity Chromatography.

Immunogène: Collagen type II purified from Human knee and Bovine nasal cartilage.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur COL2A1

Antigène: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Abstract: COL2A1 Produits

Synonymes: anticorps ANFH, anticorps AOM, anticorps COL11A3, anticorps SEDC, anticorps Col2, anticorps Col2a, anticorps Col2a-1, anticorps Del1, anticorps Dmm, anticorps Lpk, anticorps M100856, anticorps Rgsc856, anticorps CG2A1A, anticorps COLLII, anticorps col2a1a, anticorps CB11, anticorps col2a1, anticorps coll2a1, anticorps fb38c06, anticorps fc10c01, anticorps wu:fb38c06, anticorps wu:fc10c01, anticorps collagen type II alpha 1 chain, anticorps collagen, type II, alpha 1, anticorps collagen, type II, alpha 1 L homeolog, anticorps collagen, type II, alpha 1 S homeolog, anticorps collagen, type II, alpha 1a, anticorps COL2A1, anticorps Col2a1, anticorps col2a1, anticorps col2a1.L, anticorps col2a1.S, anticorps col2a1a

Sujet: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1

ID gène: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding