Vimentin anticorps (AA 400-500)

N° du produit ABIN1574034

Détail du produit anti-Vimentin anticorps

Antigène: Vimentin (VIM)

Épitope: AA 400-500

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Specificité: Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

Réactivité croisée (Details): Vimentin Antibody, pAb, Rabbit detects endogenous levels of human vimentin protein. Sequence homology predicts that it will also react with mouse and rat vimentin.

Purification: Immunoaffinity chromatography

Immunogène: Synthetic peptide (KLH-coupled) derived from within AA 400-500 of human vimentin

Isotype: IgG

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLImmunohistochemistry: 5-10 µg/mLFlow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C/-20 °C

Stockage commentaire: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin (VIM Produits)

Synonymes: anticorps CTRCT30, anticorps cb28, anticorps vime, anticorps vim, anticorps vim1, anticorps vim2, anticorps VIM, anticorps Vimentin, anticorps vim4, anticorps vimentin, anticorps vimentin L homeolog, anticorps vimentin S homeolog, anticorps VIM, anticorps Vim, anticorps vim, anticorps vim.L, anticorps vim.S

Sujet: Vimentin is a member of the intermediate filament protein family. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Despite the fact that most intermediate filaments exist as stable structures, in fibroblasts, vimentin take on a dynamic structure.Vimentin Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 400-500 of human vimentin protein (Swiss Prot: P08670).

Pathways: Caspase Cascade in Apoptosis