GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
Réactivité croisée (Details)
GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
GFP
Reactivité: Aequorea victoria
FACS, IM
Hôte: Chèvre
Polyclonal
FITC
Indications d'application
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.2-1 µg/mL Immunoprecipitation: 1-5 µg/mL Other Applications: user optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % Sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C/-20 °C
Stockage commentaire
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
McIntyre, Davis, Joiner, Williams, Tsai, Jenkins, McEwen, Zhang, Escobado, Thomas, Szymanska, Johnson, Beales, Green, Mullikin, Sabo, Muzny, Gibbs, Attié-Bitach, Yoder, Reed, Katsanis, Martens: "Gene therapy rescues cilia defects and restores olfactory function in a mammalian ciliopathy model." dans: Nature medicine, Vol. 18, Issue 9, pp. 1423-8, (2012) (PubMed).
anticorps green fluorescent protein, anticorps gfp
Classe de substances
Viral Protein
Sujet
Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y. GFP Antibody, pAb, Chicken is developed in chicken using purified recombinant full-length GFP protein.