HMGB1 anticorps

N° du produit ABIN1580427

Cet anticorps anti-HMGB1 est un anticorps Souris Monoclonal détectant HMGB1 dans WB, IHC, IF et ICC. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Porc et Mammifères.

Aperçu rapide pour HMGB1 anticorps (ABIN1580427)

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Mammifères

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMGB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1F3

Détail du produit anti-HMGB1 anticorps

Purification: affinity purified antibody

Isotype: IgG2b

Information d'application

Indications d'application: The antibody solution can be used at dilutions of 1:1,000 or higher in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:1,000-2,000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 4°C short term or -20°C long term.

Détails sur HMGB1

Antigène: HMGB1 (High Mobility Group Box 1 (HMGB1))

Autre désignation: High mobility group box 1, Amphoterin, HMGB1

Sujet: High-mobility group proteins were named originally since they are abundant relatively low molecular weight proteins which run quickly on SDS-PAGE gels. High-mobility group proteis box 1 (HMGB1) is one of these. The bx in the name refers to the so-called high mobility group (HMG) box, a compact domain involved in DNA binding and protein-protein interactions. The HMGB1 molecule has two of these HMG domains. The protein is also called amphoterin, this name being derived from the presence of two highly charged regions in the molecule, a relatively neutrally charged N-terminus and a very negatively charged C-terminus. In fact, the molecule is very unusually charged throughout, the human sequence consisting of 16.7% Glutamic acid, 9.3% Aspartic acid, 20% Lysine and 9.3% Arginine. HMGB1 can bind Toll like receptor 4 (TLR4) and the Receptor for Advanced Glycation End products (RAGE). TLRs are components of the innate immune system, first recognized as a family of receptors which recognize Pathogen Associated Molecular Pattern molecules (PAMPs). PAMPs are common components of bacteria and when TLRs bind these, a strong inflammatory response is activated. More recently it has been recognized that TLRs can also be activated by Damage Associated Molecular Pattern molecules (DAMPs), which are endogenous substances released from damaged and diseased cells which also bind to TLR family receptors and also activate inflammation. HMGB1 is such a DAMP, binding to TLR4, and much evidence suggests that HMGB1 is a strong activator of inflammation. Interestingly, HMGB1 is released by necrotic cells but not by apoptotic cells. The HGNC name for this protein is HBGB1.

Pathways: Signalisation p53, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome