Nop5p anticorps
Reactivité: Saccharomyces cerevisiae
ICC, IF, IHC, WB
Hôte: Souris
Monoclonal
34B12
unconjugated
1 image
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- Antigène
- Nop5p
- Reactivité
- Saccharomyces cerevisiae
- Hôte
- Souris
- Clonalité
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- conc. tissue culture supernatant
- Stérilité
- Sterile filtered
- Clone
- 34B12
- Isotype
- IgG3
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- Indications d'application
- For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µL for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/mL.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store at 4°C short term or -20°C long term.
-
- Antigène
- Nop5p
- Sujet
- Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).
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