MAPK6 anticorps (Internal Region)

N° du produit ABIN1590009

Cet anticorps anti-MAPK6 est un anticorps Chèvre Polyclonal détectant MAPK6 dans WB, ELISA, IHC et IP. Adapté pour Humain et Souris.

Aperçu rapide pour MAPK6 anticorps (Internal Region) (ABIN1590009)

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK6 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-MAPK6 anticorps

Épitope: Internal Region

Fonction: ERK3 / MAPK6

Séquence: HSPVGSPLKS IQ

Specificité: Reported variants represent identical protein (NP_056621.4, NP_081694.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Western Blot: In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11926. Western blots of the IP were

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur MAPK6

Antigène: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Autre désignation: Mapk6

Sujet: MAPK6, mitogen-activated protein kinase 6, ERK3, HsT17250, PRKM6, p97MAPK, ERK-3, MAP kinase 6, MAP kinase isoform p97, MAPK 6, extracellular signal-regulated kinase 3, extracellular signal-regulated kinase, p97, p97-MAPK, protein kinase, mitogen-activate

ID gène: 5597, 50772, 58840

NCBI Accession: NP_002739

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C