CA1 anticorps (Biotin)

N° du produit ABIN1607668

Détail du produit anti-CA1 anticorps (Biotin)

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CA1 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity against Carbonic Anhydrase I from other sources may occur but has not been specifically determined.

Pureté: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Carbonic Anhydrase I [Human Erythrocytes]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody has been assayed against 1.0 µg of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur CA1

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Autre désignation: Carbonic Anhydrase I (CA1 Produits)

Synonymes: anticorps CA-I, anticorps CAB, anticorps Car1, anticorps AW555628, anticorps Ca1, anticorps Car-1, anticorps BG:DS00941.1, anticorps CAH, anticorps CG7820, anticorps Dmel\\CG7820, anticorps Gh7, anticorps ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, anticorps ATBCA1, anticorps ATSABP3, anticorps BETA CARBONIC ANHYDRASE 1, anticorps F4P13.5, anticorps F4P13_5, anticorps SABP3, anticorps SALICYLIC ACID-BINDING PROTEIN 3, anticorps carbonic anhydrase 1, anticorps CA1, anticorps LOC100135826, anticorps GB15888, anticorps carbonic anhydrase 1, anticorps Carbonic anhydrase 1, anticorps carbonic anhydrase I, anticorps carbonic anhydrase, anticorps CA1, anticorps Car1, anticorps CAH1, anticorps LOC100135826, anticorps LOC408827, anticorps Tsp_05114, anticorps LOC100153915, anticorps LOC100050192, anticorps Ca1

Sujet: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic Anhydrase 1 (CA1) is closely related to Carbonic Anhydrase 2 (CA2) and Carbonic Anhydrase 3 (CA3), and it is a cytosolic protein that is found at the highest level in erythrocytes. Anti-Carbonic anhydrase I antibody is ideal for investigators involved in serum protein components. Synonyms: Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

ID gène: 759

UniProt: P00915